A DNAzyme-mediated signal amplification biosensor for ultrasensitive detection of lead ions based on SERS tags
نویسندگان
چکیده
A trace lead ion (Pb 2+ ) detection platform combining surface-enhanced Raman spectroscopy (SERS) with nucleic acid cycle multiple signal amplification.
منابع مشابه
A SERS DNAzyme biosensor for lead ion detection.
SERS biosensor for sensitive and selective detection of lead ions (Pb(2+)) based on DNAzyme was developed by taking advantage of the specific catalytic reaction of DNAzyme upon binding to Pb(2+) ions. Detection was accomplished by SERS nanoprobe labeled with DNA and Raman reporters for signal amplification.
متن کاملA target-triggered exponential amplification-based DNAzyme biosensor for ultrasensitive detection of folate receptors.
We develop a new method for ultrasensitive detection of folate receptors (FRs) using a target-triggered isothermally exponential amplification reaction (EXPAR)-based DNAzyme biosensor. This method exhibits excellent specificity and high sensitivity with a detection limit as low as 0.23 fM and a large dynamic range of 6 orders of magnitude from 1 fM to 1 nM. It might be further applied for the d...
متن کاملA sensitive biosensor with a DNAzyme for lead(II) detection based on fluorescence turn-on.
In this paper, we described a new DNAzyme-based fluorescent biosensor for the detection of Pb(2+). In the biosensor, the bulged structure is formed between the substrate labeled with fluorescein amidite (FAM) and DNAzyme after being annealed. Ethidium bromide (EB), the DNA intercalator, then intercalates into the double-stranded DNA section. Once FAM is excited, the FRET takes place from FAM to...
متن کاملHighly sensitive detection of telomerase using a telomere-triggered isothermal exponential amplification-based DNAzyme biosensor.
A telomere-triggered isothermal exponential amplification-based DNAzyme biosensor is developed for highly sensitive detection of telomerase in cancer cells even at the single-cell level. This biosensor can be further applied for the screening of telomerase inhibitors for anticancer drug development.
متن کاملAn ultrasensitive DNAzyme-based colorimetric strategy for nucleic acid detection.
An ultrasensitive DNAzyme-based colorimetric DNA detection strategy was developed by utilizing gold nanoparticles modified with both peroxidase-mimicking DNAzymes and capture DNAs and magnetic nanoparticles modified with another capture DNAs, with a detection limit for a model Chlamydia gene of 50 fM.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Sensors & diagnostics
سال: 2023
ISSN: ['2635-0998']
DOI: https://doi.org/10.1039/d2sd00147k